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Tert-butylcarbazate is often used as an intermediate in organic synthesis. It can be used in the preparation of various carbazone derivatives, which are used in medicinal chemistry and as analytical reagents. Tert-butylcarbazate can also be used as a building block for the synthesis of various organic compounds, such as agrochemicals and specialty chemicals.

In addition, tert-butylcarbazate can be used in the protection of carbonyl compounds in organic synthesis. It can react with aldehydes and ketones to form carbazone derivatives, which can protect the carbonyl group from unwanted reactions. This protection strategy is widely used in organic synthesis for the selective functionalization of carbonyl compounds in complex molecules.

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