Liquimax Triglyceride-SLR (TBHBA/10 Minutes End Point) GPO – PAP TRINDER’S METHOD

: Triglycerides are esters of the trihydric alcohol glycerol with3 Iong chain fatty acids. They are partly synthesized in the liver and partly ingested in food. The determination of triglycerides is utilized in the diagnosis and treatment of patients having diabetes mellitus, nephrosis, liver obstruction, lipid metabolism disorders and numerous other endocrine diseases. The enzymatic triglycerides assay as described by Eggstein and Kreutz still required saponification with potassium hydroxide. Numerous attempts were subsequently made to replace alkaline saponification by enzymatic hydrolysis with lipase. Bucolo and David tested a lipase/protease mixture; Wahlefeld used an esterase from the liver in combination with a particularly effective lipase from Rhizopus arrhizus for hydrolysis. This method is based on the work by Wahlefeld using a lipoprotein lipase from microorganisms for the rapid and complete hydrolysis of triglycerides to glycerol followed by oxidation to dihydroxyacetone phosphate and hydrogen peroxide. The hydrogen peroxide produced then reacts with 4- aminophenazone and TBHBAunder the catalytic action of peroxidase to form a red complex (Trinder endpoint reaction).