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Liquimax CK-NAC

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INTENDED USE: For the quantitative determination of Creatine Kinase (CK) activity in human serum.

 

Creatine kinase Plays an important role in the energy-storing mechanism of tissue by Catalyzing the reversible reaction between creatine and ATP to form creatine phosphate and ADP. Creatine kinase (CK) is an enzyme which is found primarily in skeletal muscle, cardiac muscle and brain tissue.Elevated levels of CK are associated with myocardial infarction, various muscle disorders and diseases such as progressive Duchenne-type muscular dystrophy. In myocardial infarction, peak CK levels occur 24 to 36 hours after onset of chest pain and depending on the extent of damage can reach more than 10 times normal levels.

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Description

The CK Reagent is a modification of the Szasz procedure. CK reversibly catalyzes the transfer of a phosphate group from creatine phosphate to adenosine triphosphate (ATP) as products. The ATP formed is used to produce glucose-6- phosphate and ADP from glucose. This reaction is catalyzed by hexokinase (HK) which requires magnesium ions for maximum activity. The glucose-6 phosphate is oxidized by the action of the enzyme glucose-6 phosphate dehydrogenase (G-6-PDH) with simultaneous reduction of the coenzyme nicotinamide adenine dinucleotide phosphate (NADP) to give NADPH and 6-phosphogluconate. The rate of increase of absorbance at 340/380 nm due to the formation of NADPH is directly proportional to the activity of the CK in the sample.

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  • Store Name: Avecon Healthcare
  • Vendor: Avecon Healthcare
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